Photonic neural probe enabled microendoscopes for light-sheet light-field computational fluorescence brain imaging.

ABSTRACT:

Significance

Light-sheet fluorescence microscopy is widely used for high-speed, high-contrast, volumetric imaging. Application of this technique to in vivo brain imaging in non-transparent organisms has been limited by the geometric constraints of conventional light-sheet microscopes, which require orthogonal fluorescence excitation and collection objectives. We have recently demonstrated implantable photonic neural probes that emit addressable light sheets at depth in brain tissue, miniaturizing the excitation optics. Here, we propose a microendoscope consisting of a light-sheet neural probe packaged together with miniaturized fluorescence collection optics based on an image fiber bundle for lensless, light-field, computational fluorescence imaging.

Aim

Foundry-fabri

SUBMITTER: Ding P 

PROVIDER: S-EPMC10846542 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature