Identification of cow milk epitopes to characterize and quantify disease-specific T cells in allergic children.

ABSTRACT:

Background

Cow milk (CM) allergy is the most prevalent food allergy in young children in the United States and Great Britain. Current diagnostic tests are either unreliable (IgE test and skin prick test) or resource-intensive with risks (food challenges).

Objective

We sought to determine whether allergen-specific T cells in CM-allergic (CMA) patients have a distinct quality and/or quantity that could potentially be used as a diagnostic marker.

Methods

Using PBMCs from 147 food-allergic pediatric subjects, we mapped T-cell responses to a set of reactive epitopes in CM that we compiled in a peptide pool. This pool induced cytokine responses in in vitro cultured cells distinguishing subjects with CMA from subjects without CMA. We further used the pool to isolate and char

SUBMITTER: Lewis SA 

PROVIDER: S-EPMC10846667 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature