SARM1 regulates NAD⁺-linked metabolism and select immune genes in macrophages.

ABSTRACT: Sterile alpha and HEAT/armadillo motif-containing protein (SARM1) was recently described as a NAD⁺-consuming enzyme and has previously been shown to regulate immune responses in macrophages. Neuronal SARM1 is known to contribute to axon degeneration due to its NADase activity. However, how SARM1 affects macrophage metabolism has not been explored. Here, we show that macrophages from Sarm1^-/- mice display elevated NAD⁺ concentrations and lower cyclic ADP-ribose, a known product of SARM1-dependent NAD⁺ catabolism. Further, SARM1-deficient macrophages showed an increase in the reserve capacity of oxidative phosphorylation and glycolysis compared to WT cells. Stimulation of macrophages to a proinflammatory state by lipopolysaccharide (LPS) revealed t

SUBMITTER: Shanahan KA 

PROVIDER: S-EPMC10847163 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature