Intragenic Agrobacterium-mediated gene transfer mimics micro-translocations without foreign DNA.

ABSTRACT:

Main conclusion

Agrobacterium-mediated transformation of Nicotiana tabacum, using an intragenic T-DNA region derived entirely from the N. tabacum genome, results in the equivalence of micro-translocations within genomes. Intragenic Agrobacterium-mediated gene transfer was achieved in Nicotiana tabacum using a T-DNA composed entirely of N. tabacum DNA, including T-DNA borders and the acetohydroxyacid synthase gene conferring resistance to sulfonylurea herbicides. Genomic analysis of a resulting plant, with single locus inheritance of herbicide resistance, identified a single insertion of the intragenic T-DNA on chromosome 5. The insertion event was composed of three N. tabacum DNA fragments from other chromosomes, as assembled on the T-DNA vector. This validates that intragenic tran

SUBMITTER: Barrell PJ 

PROVIDER: S-EPMC10847175 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature