Lectin-mediated, time-efficient, and high-yield sorting of different morphologically intact nephron segments.

ABSTRACT: The kidney is a highly complex organ equipped with a multitude of miniscule filter-tubule units called nephrons. Each nephron can be subdivided into multiple segments, each with its own morphology and physiological function. To date, conventional manual approaches to isolate specific nephron segments are very laborious, time-consuming, often limited to only a specific segment, and typically have low yield. Here, we describe a novel, unconventional method that is superior in many aspects to previous protocols by combining low-cost fluorophore-conjugated lectins or agglutinins (Flaggs) with flow sorting. This allows the simultaneous separation of different nephron segments with preserved 3D morphology from mouse or human samples in under 3 h. Using a 200-µm nozzle and 5 psi, glomeruli, proxi

SUBMITTER: Roskosch J 

PROVIDER: S-EPMC10847228 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature