The antibacterial activity and mechanism of a novel peptide MR-22 against multidrug-resistant Escherichia coli.

ABSTRACT:

Introduction

Bacterial infections have become serious threats to human health, and the excessive use of antibiotics has led to the emergence of multidrug-resistant (MDR) bacteria. E. coli is a human bacterial pathogen, which can cause severe infectious. Antimicrobial peptides are considered the most promising alternative to traditional antibiotics.

Materials and methods

The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and hemolytic activity were determined by the microdilution method. The antimicrobial kinetics of MR-22 against E. coli were studied by growth curves and time-killing curves. The cytotoxicity of MR-22 was detected by the CCK-8 assay. The antimicrobial activity of MR-22 in salt, serum, heat and trypsin was determ

SUBMITTER: Tian C 

PROVIDER: S-EPMC10847306 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature