Recording ten-fold larger I_Kr conductances with automated patch clamping using equimolar Cs⁺ solutions.

ABSTRACT: Background: The rapid delayed rectifier potassium current (I_Kr) is important for cardiac repolarization and is most often involved in drug-induced arrhythmias. However, accurately measuring this current can be challenging in human-induced pluripotent stem cell (hiPSC)-derived cardiomyocytes because of its small current density. Interestingly, the ion channel conducting I_Kr, hERG channel, is not only permeable to K⁺ ions but also to Cs⁺ ions when present in equimolar concentrations inside and outside of the cell. Methods: In this study, I_hERG was measured from Chinese hamster ovary (CHO)-hERG cells and hiPSC-CM using either Cs⁺ or K⁺ as the charge carrier. Equimolar Cs⁺ has been used in the

SUBMITTER: Bloothooft M 

PROVIDER: S-EPMC10847579 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature