In vitro culture and tissue-derived specific expression of melanocytes from ovary of adult Silky Fowl.

ABSTRACT: The presence of a significant number of melanocytes in the ovary and follicular membrane of Silky Fowl suggests their potential involvement in follicle development. Currently, there is a lack of available data regarding to the isolation of primary melanocytes from adult chickens. To date, primary melanocytes and their in vitro culture system have been successfully conducted in the peritoneum of chicken embryos. Herein, melanocytes from silky fowl ovaries were isolated and identified. Silky Fowl ovaries were obtained by mixed digestion of 0.1% collagenase II and 0.25% trypsin-EDTA. Melanocytes could be further purified and cultured up to 5 generations in vitro. RNA-seq analysis was used to investigate whether there were differences in the functional status of melanocytes in different tissue

SUBMITTER: Tai Y 

PROVIDER: S-EPMC10847685 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature