All-potassium channel CRISPR screening reveals a lysine-specific pathway of insulin secretion.

ABSTRACT:

Objective

Genome-scale CRISPR-Cas9 knockout coupled with single-cell RNA sequencing (scRNA-seq) has been used to identify function-related genes. However, this method may knock out too many genes, leading to low efficiency in finding genes of interest. Insulin secretion is controlled by several electrophysiological events, including fluxes of K_ATP depolarization and K⁺ repolarization. It is well known that glucose stimulates insulin secretion from pancreatic β-cells, mainly via the K_ATP depolarization channel, but whether other nutrients directly regulate the repolarization K⁺ channel to promote insulin secretion is unknown.

Methods

We used a system involving CRISPR-Cas9-mediated knockout of all 83 K⁺ channels and scRNA-

SUBMITTER: Lu J 

PROVIDER: S-EPMC10847698 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature