Proteomic and metabolomic analyses illustrate the mechanisms of expression of the O⁶ -methylguanine-DNA methyltransferase gene in glioblastoma.

ABSTRACT:

Aim

Glioblastoma (GBM) has been reported to be the most common high-grade primary malignant brain tumor in clinical practice and has a poor prognosis. O⁶ -methylguanine-DNA methyltransferase (MGMT) promoter methylation has been related to prolonged overall survival (OS) in GBM patients after temozolomide treatment.

Methods

Proteomics and metabolomics were combined to explore the dysregulated metabolites and possible protein expression alterations in white matter (control group), MGMT promoter unmethylated GBM (GBM group) or MGMT promoter methylation positive GBM (MGMT group).

Results

In total, 2745 upregulated and 969 downregulated proteins were identified in the GBM group compared to the control group, and 131 upregulated and 299 downregulated proteins wer

SUBMITTER: Chen X 

PROVIDER: S-EPMC10848106 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature