Comparative genomic analysis of uropathogenic Escherichia coli strains from women with recurrent urinary tract infection.

ABSTRACT:

Introduction

Recurrent urinary tract infections (RUTIs) caused by uropathogenic Escherichia coli are costly public health problems impacting patients' quality of life.

Aim

In this work, a comparative genomics analysis of three clinical RUTI strains isolated from bladder biopsy specimens was performed.

Materials and methods

One hundred seventy-two whole genomes of urinary tract E. coli strains were selected from the NCBI database. The search for virulence factors, fitness genes, regions of interest, and genetic elements associated with resistance was manually carried out. The phenotypic characterization of antibiotic resistance, haemolysis, motility, and biofilm formation was performed. Moreover, adherence and invasion assays with human bladder HTB-5 cell

SUBMITTER: Flores-Oropeza MA 

PROVIDER: S-EPMC10848155 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature