Chromosome-length genome assembly and karyotype of the endangered black-footed ferret (Mustela nigripes).

ABSTRACT: The black-footed ferret (Mustela nigripes) narrowly avoided extinction to become an oft-cited example of the benefits of intensive management, research, and collaboration to save a species through ex situ conservation breeding and reintroduction into its former range. However, the species remains at risk due to possible inbreeding, disease susceptibility, and multiple fertility challenges. Here, we report the de novo genome assembly of a male black-footed ferret generated through a combination of linked-read sequencing, optical mapping, and Hi-C proximity ligation. In addition, we report the karyotype for this species, which was used to anchor and assign chromosome numbers to the chromosome-length scaffolds. The draft assembly was ~2.5 Gb in length, with 95.6% of it anchored to 19 chromoso

SUBMITTER: Kliver S 

PROVIDER: S-EPMC10848218 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature