Single-cell RNA sequencing of mid-to-late stage spider embryos: new insights into spider development.

ABSTRACT:

Background

The common house spider Parasteatoda tepidariorum represents an emerging new model organism of arthropod evolutionary and developmental (EvoDevo) studies. Recent technical advances have resulted in the first single-cell sequencing (SCS) data on this species allowing deeper insights to be gained into its early development, but mid-to-late stage embryos were not included in these pioneering studies.

Results

Therefore, we performed SCS on mid-to-late stage embryos of Parasteatoda and characterized resulting cell clusters by means of in-silico analysis (comparison of key markers of each cluster with previously published information on these genes). In-silico prediction of the nature of each cluster was then tested/verified by means of additional in-situ hybridization

SUBMITTER: Medina-Jimenez BI 

PROVIDER: S-EPMC10848406 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature