Developing the Protocol Infrastructure for DNA Sequencing Natural History Collections.

ABSTRACT: Intentionally preserved biological material in natural history collections represents a vast repository of biodiversity. Advances in laboratory and sequencing technologies have made these specimens increasingly accessible for genomic analyses, offering a window into the genetic past of species and often permitting access to information that can no longer be sampled in the wild. Due to their age, preparation and storage conditions, DNA retrieved from museum and herbarium specimens is often poor in yield, heavily fragmented and biochemically modified. This not only poses methodological challenges in recovering nucleotide sequences, but also makes such investigations susceptible to environmental and laboratory contamination. In this paper, we review the practical challenges associated with ma

SUBMITTER: Ferrari G 

PROVIDER: S-EPMC10848826 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature