How to Detect Antibodies Against Babesia divergens in Human Blood Samples.

ABSTRACT:

Background

Today only indirect fluorescent antibody assays (IFAs) are commercially available to detect antibodies against Babesia divergens in humans. IFA is subjective and requires highly experienced staff. We have therefore developed an enzyme-linked immunosorbent assay (ELISA)-based method for measuring anti-B. divergens immunoglobulin G antibodies in human blood samples.

Methods

Crude merozoite extract from in vitro cultures of a new B. divergens isolate was used in ELISA to detect antibodies in different sets of samples: Borrelia burgdorferi-positive samples, healthy individuals, tick-bitten individuals including follow-up samples 3 months later, positive control samples from patients with an active Babesia infection, and samples from

SUBMITTER: Tijani MK 

PROVIDER: S-EPMC10849114 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature