Automated single-cell proteomics providing sufficient proteome depth to study complex biology beyond cell type classifications.

ABSTRACT: Mass spectrometry (MS)-based single-cell proteomics (SCP) has gained massive attention as a viable complement to other single cell approaches. The rapid technological and computational advances in the field have pushed the boundaries of sensitivity and throughput. However, reproducible quantification of thousands of proteins within a single cell at reasonable proteome depth to characterize biological phenomena remains a challenge. To address some of those limitations we present a combination of fully automated single cell sample preparation utilizing a dedicated chip within the picolitre dispensing robot, the cellenONE. The proteoCHIP EVO 96 can be directly interfaced with the Evosep One chromatographic system for in-line desalting and highly reproducible separation with a throughput of 80

SUBMITTER: Ctortecka C 

PROVIDER: S-EPMC10849471 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature