Histone proteoform analysis reveals epigenetic changes in adult mouse brown adipose tissue in response to cold stress.

ABSTRACT: Regulation of the thermogenic response by brown adipose tissue (BAT) is an important component of energy homeostasis with implications for the treatment of obesity and diabetes. Our preliminary analyses uncovered many nodes representing epigenetic modifiers that are altered in BAT in response to chronic thermogenic activation. Thus, we hypothesized that chronic thermogenic activation broadly alters epigenetic modifications of DNA and histones in BAT. Motivated to understand how BAT function is regulated epigenetically, we developed a novel method for the first-ever unbiased top-down proteomic quantitation of histone modifications in BAT and validated our results with a multi-omic approach. To test our hypothesis, wildtype male C57BL/6J mice were housed under chronic conditions of thermoneu

SUBMITTER: Taylor BC 

PROVIDER: S-EPMC10849524 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature