High-Resolution Laminar Identification in Macaque Primary Visual Cortex Using Neuropixels Probes.

ABSTRACT: Laminar electrode arrays allow simultaneous recording of activity of many cortical neurons and assignment to layers using current source density (CSD) analyses. Electrode arrays with 100-micron contact spacing have been used to estimate borders between layer 4 versus superficial or deep layers, but in macaque primary visual cortex (V1) there are far more layers, such as 4A which is only 50-100 microns thick. Neuropixels electrode arrays have 20-micron spacing, and thus could potentially discern thinner layers and more precisely identify laminar borders. Here we show that laminar distributions of CSDs lack consistency and the spatial resolution required for thin layers and accurate layer boundaries. To take full advantage of high density Neuropixels arrays, we have developed approaches base

SUBMITTER: Zhang LA 

PROVIDER: S-EPMC10849622 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature