Harnessing axonal transport to map reward circuitry: Differing brain-wide projections from medial forebrain domains.

ABSTRACT: Neurons project long axons that contact other distant neurons. Projections can be mapped by hijacking endogenous membrane trafficking machinery by introducing tracers. To witness functional connections in living animals, we developed a tracer detectible by magnetic resonance imaging (MRI), Mn(II). Mn(II) relies on kinesin-1 and amyloid-precursor protein to travel out axons. Within 24h, projection fields of cortical neurons can be mapped brain-wide with this technology. MnCl₂ was stereotactically injected either into anterior cingulate area (ACA) or into infralimbic/prelimbic (IL/PL) of medial forebrain (n=10-12). Projections were imaged, first by manganese-enhanced MRI (MEMRI) live, and then after fixation by microscopy. MR images were collected at 100μm isotropic resolution (~5

SUBMITTER: Bearer EL 

PROVIDER: S-EPMC10849663 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature