Exploring the intratumoral heterogeneity of DNA ploidy in prostate cancer.

ABSTRACT:

Background

Prostate cancer is morphologically and molecularly heterogeneous. Genomic heterogeneity might be mirrored by variability in DNA ploidy. Aneuploidy is a hallmark of genomic instability and associated with tumor aggressiveness. Little attention has been paid to the biological significance of the diploid tumor cell population that often coexists with aneuploid populations. Here, we investigated the role of DNA ploidy in tumor heterogeneity and clonal evolution.

Methods

Three radical prostatectomy specimens with intratumoral heterogeneity based on nuclear features on H&E were selected. DNA content of each subpopulation was determined by DNA image cytometry and silver in situ hybridization (SISH). Genomic evolution was inferred from array comparative genomic hybridizat

SUBMITTER: Vlajnic T 

PROVIDER: S-EPMC10849929 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature