Subcutaneous adipose tissue dopamine D2 receptor is increased in prediabetes and T2D.

ABSTRACT:

Purpose

To evaluate the dopaminergic signaling in human adipose tissue in the context of obesity and type 2 diabetes (T2D) and potential direct implications in adipose tissue metabolism.

Methods

mRNA and protein expression of dopamine receptors D1 and D2 (DRD1 and DRD2) were determined in subcutaneous adipose tissue from subjects without or with T2D and with different body weight, and correlated with markers of obesity, hyperglycemia, and insulin resistance. Glucose uptake and lipolysis were measured in adipocytes ex vivo following short-term exposure to dopamine, DRD1 receptor agonist (SKF81297), or DRD2 receptor agonist (bromocriptine).

Results

DRD1 and DRD2 gene expression in subcutaneous adipose tissue correlated positively with clinical markers of insulin resista

SUBMITTER: Vranic M 

PROVIDER: S-EPMC10850013 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature