Comparison of LC-MS-based methods for the determination of carboxylic acids in animal matrices.

ABSTRACT: Carboxylic acids (CAs) are key players in human and animal metabolism. As they are hardly retained under reversed-phase liquid chromatography (RP-LC) conditions in their native form, derivatization is an option to make them accessible to RP-LC and simultaneously increase their response for mass spectrometric detection. In this work, two RP-LC tandem mass spectrometry-based methods using aniline or 3-nitrophenylhydrazine (3-NPH) as derivatization agents were compared with respect to several factors including completeness of derivatization, apparent recoveries (R_As) in both cow feces and ruminal fluid, and concentrations obtained in feces and ruminal fluid of cows. Anion exchange chromatography coupled to high-resolution mass spectrometry (AIC-HR-MS) served as reference method. De

SUBMITTER: Schwartz-Zimmermann HE 

PROVIDER: S-EPMC10850028 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature