Combinatorial optimization of gene expression through recombinase-mediated promoter and terminator shuffling in yeast.

ABSTRACT: Microbes are increasingly employed as cell factories to produce biomolecules. This often involves the expression of complex heterologous biosynthesis pathways in host strains. Achieving maximal product yields and avoiding build-up of (toxic) intermediates requires balanced expression of every pathway gene. However, despite progress in metabolic modeling, the optimization of gene expression still heavily relies on trial-and-error. Here, we report an approach for in vivo, multiplexed Gene Expression Modification by LoxPsym-Cre Recombination (GEMbLeR). GEMbLeR exploits orthogonal LoxPsym sites to independently shuffle promoter and terminator modules at distinct genomic loci. This approach facilitates creation of large strain libraries, in which expression of every pathway gene ranges over 120

SUBMITTER: Cautereels C 

PROVIDER: S-EPMC10850122 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature