Regulation of PDGFRα⁺ cells and ICC in progesterone-mediated slow colon transit in pregnant mice.

ABSTRACT:

Background

Progesterone can inhibit intestinal smooth muscle contraction; however, the specific mechanism remains unclear. Besides smooth muscle cells, smooth muscle has two important mesenchymal cells, namely interstitial cells of Cajal (ICC) and PDGFRα⁺ cells, which induce the contraction and relaxation of smooth muscles. We aimed to explore the regulation of PDGFRα⁺ cells and ICC in progesterone-mediated colon slow transit in pregnant mice.

Methods

Colon transit experiments were performed in vivo and in vitro to observe slow colon transit. The expression of PDGFRα and c-KIT was detected by Western blot, RT-PCR, and immunofluorescence. An isometric tension experiment was performed to investigate smooth muscle contractions.

Results

SUBMITTER: Lu C 

PROVIDER: S-EPMC10850515 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature