Identification of RNA silencing suppressor encoded by citrus chlorotic dwarf-associated virus.

ABSTRACT:

Introduction

Citrus chlorotic dwarf-associated virus (CCDaV) is an economically important citrus virus associated with leaf curling, deformation, and chlorosis found in China. Plants have evolved RNA silencing to defend against viral infections; however, the mechanism by which CCDaV suppresses RNA silencing in citrus remains unknown.

Methods

Six proteins encoded by CCDaV were ectopically expressed in Nicotiana benthamiana 16c using the pCHF3 vector to identify RNA-silencing suppression activities.

Results

V2 protein encoded by CCDaV suppressed local RNA silencing and systemic RNA silencing triggered by GFP RNA, but did not impede short-distance movement of the RNA silencing signal in N. benthamiana 16c. GFP fluorescence observations showed that the abili

SUBMITTER: Ye X 

PROVIDER: S-EPMC10850569 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature