Sfrp1 inhibits lung fibroblast invasion during transition to injury-induced myofibroblasts.

ABSTRACT:

Background

Fibroblast-to-myofibroblast conversion is a major driver of tissue remodelling in organ fibrosis. Distinct lineages of fibroblasts support homeostatic tissue niche functions, yet their specific activation states and phenotypic trajectories during injury and repair have remained unclear.

Methods

We combined spatial transcriptomics, multiplexed immunostainings, longitudinal single-cell RNA-sequencing and genetic lineage tracing to study fibroblast fates during mouse lung regeneration. Our findings were validated in idiopathic pulmonary fibrosis patient tissues in situ as well as in cell differentiation and invasion assays using patient lung fibroblasts. Cell differentiation and invasion assays established a function of SFRP1 in regulating human lung fibroblas

SUBMITTER: Mayr CH 

PROVIDER: S-EPMC10850614 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature