IL-20 controls resolution of experimental colitis by regulating epithelial IFN/STAT2 signalling.

ABSTRACT:

Objective

We sought to investigate the role of interleukin (IL)-20 in IBD and experimental colitis.

Design

Experimental colitis was induced in mice deficient in components of the IL-20 and signal transducer and activator of transcription (STAT)2 signalling pathways. In vivo imaging, high-resolution mini-endoscopy and histology were used to assess intestinal inflammation. We further used RNA-sequencing (RNA-Seq), RNAScope and Gene Ontology analysis, western blot analysis and co-immunoprecipitation, confocal microscopy and intestinal epithelial cell (IEC)-derived three-dimensional organoids to investigate the underlying molecular mechanisms. Results were validated using samples from patients with IBD and non-IBD control subjects by a combination of RNA-Seq, organoids and immun

SUBMITTER: Chiriac MT 

PROVIDER: S-EPMC10850655 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature