Characterisation of T cell receptor repertoires in coeliac disease.

ABSTRACT:

Aims

Characterise T-cell receptor gene (TR) repertoires of small intestinal T cells of patients with newly diagnosed (active) coeliac disease (ACD), refractory CD type I (RCD I) and patients with CD on a gluten-free diet (GFD).

Methods

Next-generation sequencing of complementarity-determining region 3 (CDR3) of rearranged T cell receptor β (TRB) and γ (TRG) genes was performed using DNA extracted from intraepithelial cell (IEC) and lamina propria cell (LPC) fractions and a small subset of peripheral blood mononuclear cell (PBMC) samples obtained from CD and non-CD (control) patients. Several parameters were assessed, including relative abundance and enrichment.

Results

TRB and TRG repertoires of CD IEC and LPC samples demonstrated lower clonality but higher frequency

SUBMITTER: Lee LW 

PROVIDER: S-EPMC10850686 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature