EP300 restores the glycolytic activity and anti-tumor function of CD8⁺ cytotoxic T cells in nasopharyngeal carcinoma.

ABSTRACT: Competition for glucose may metabolically limit T cells during cancer progression. This study shows that culturing in the condition medium (CM) of NPC c6661 cells restricted glycolytic and immune activities of CD8⁺ T cells. These cells also exhibited limited tumor-eliminating effects in mouse xenograft tumor models. Glucose supplementation restored glycolysis and immune activity of CD8⁺ T cells in vitro and in vivo by rescuing the expression of E1A binding protein p300 (EP300). EP300 upregulated bromodomain PHD finger transcription factor (BPTF) expression by catalyzing H3K27ac modification, and BPTF further activated AT-rich interaction domain 1A (ARID1A) transcription. Either BPTF or ARID1A knockdown in CD8⁺ T cells reduced their glycolytic

SUBMITTER: Xia Z 

PROVIDER: S-EPMC10850748 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature