HetMM: A Michaelis-Menten model for non-homogeneous enzyme mixtures.

ABSTRACT: The Michaelis-Menten model requires its reaction velocities to come from a preparation of homogeneous enzymes, with identical or near-identical catalytic activities. However, this condition is not always met. We introduce a kinetic model that relaxes this requirement, by assuming there are an unknown number of enzyme species drawn from a probability distribution whose standard deviation is estimated. Through simulation studies, we demonstrate the method accurately discriminates between homogeneous and heterogeneous data, even with moderate levels of experimental error. We applied this model to three homogeneous and three heterogeneous biological systems, showing that the standard and heterogeneous models outperform respectively. Lastly, we show that heterogeneity is not readily distinguish

SUBMITTER: Douglas J 

PROVIDER: S-EPMC10850774 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature