Spatial and single-nucleus transcriptomics decoding the molecular landscape and cellular organization of avian optic tectum.

ABSTRACT: The avian optic tectum (OT) has been studied for its diverse functions, yet a comprehensive molecular landscape at the cellular level has been lacking. In this study, we applied spatial transcriptome sequencing and single-nucleus RNA sequencing (snRNA-seq) to explore the cellular organization and molecular characteristics of the avian OT from two species: Columba livia and Taeniopygia guttata. We identified precise layer structures and provided comprehensive layer-specific signatures of avian OT. Furthermore, we elucidated diverse functions in different layers, with the stratum griseum periventriculare (SGP) potentially playing a key role in advanced functions of OT, like fear response and associative learning. We characterized detailed neuronal subtypes and identified a popu

SUBMITTER: Liao K 

PROVIDER: S-EPMC10850779 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature