Characterizing Foxp3⁺ and Foxp3^- T cells in the homeostatic state and after allo-activation: resting CD4⁺Foxp3⁺ Tregs have molecular characteristics of activated T cells.

ABSTRACT: Due to the intracellular expression of Foxp3 it is impossible to purify viable Foxp3⁺ cells on the basis of Foxp3 staining. Consequently CD4⁺Foxp3⁺ regulatory T cells (Tregs) in mice have mostly been characterized using CD4⁺CD25⁺ T cells or GFP-Foxp3 reporter T cells. However, these two populations cannot faithfully represent Tregs as the expression of CD25 and Foxp3 does not completely overlap and GFP⁺Foxp3⁺ reporter T cells have been reported to be functionally altered. The aim of this study was to characterize normal Tregs without separating Foxp3⁺ and Foxp3^- cells for the expression of the main functional molecules and proliferation behaviors by flow cytometry and to examine their gene ex

SUBMITTER: Liu Z 

PROVIDER: S-EPMC10850883 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature