HDAC9-mediated calmodulin deacetylation induces memory impairment in Alzheimer's disease.

ABSTRACT:

Aims

Alzheimer's disease (AD) is a neurodegenerative disease characterized by progressive cognitive dysfunction and memory impairment. AD pathology involves protein acetylation. Previous studies have mainly focused on histone acetylation in AD, however, the roles of nonhistone acetylation in AD are less explored.

Methods

The protein acetylation and expression levels were detected by western blotting and co-immunoprecipitation. The stoichiometry of acetylation was measured by home-made and site-specific antibodies against acetylated-CaM (Ac-CaM) at K22, K95, and K116. Hippocampus-dependent learning and memory were evaluated by using the Morris water maze, novel object recognition, and contextual fear conditioning tests.

Results

We showed that calmodulin (CaM) acetylati

SUBMITTER: Zhang HL 

PROVIDER: S-EPMC10850929 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature