Oxidative Stress Contributes to Slit Diaphragm Defects Caused by Disruption of Endocytosis.

ABSTRACT:

Introduction

Podocyte slit diaphragms are an important component of the glomerular filtration barrier. Podocyte injury frequently includes defects in slit diaphragms, and various mechanisms for these defects have been described, including altered endocytic trafficking of slit diaphragm proteins or oxidative stress. However, the potential relationship between endocytosis and oxidative stress in the context of slit diaphragm integrity has not been extensively considered.

Methods

To examine the potential relationships between endocytosis, oxidative stress, and slit diaphragm integrity, we induced genetic or pharmacological disruption of endocytosis in Drosophila nephrocytes (the insect orthologue of podocytes) and cultured human podocytes. We then employed immunofluoresc

SUBMITTER: Xi G 

PROVIDER: S-EPMC10851022 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature