Full Mass Range ΦSDM Orbitrap Mass Spectrometry for DIA Proteome Analysis.

ABSTRACT: Optimizing data-independent acquisition methods for proteomics applications often requires balancing spectral resolution and acquisition speed. Here, we describe a real-time full mass range implementation of the phase-constrained spectrum deconvolution method (ΦSDM) for Orbitrap mass spectrometry that increases mass resolving power without increasing scan time. Comparing its performance to the standard enhanced Fourier transformation signal processing revealed that the increased resolving power of ΦSDM is beneficial in areas of high peptide density and comes with a greater ability to resolve low-abundance signals. In a standard 2 h analysis of a 200 ng HeLa digest, this resulted in an increase of 16% in the number of quantified peptides. As the acquisition speed becomes even more important

SUBMITTER: Steigerwald S 

PROVIDER: S-EPMC10851225 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature