Neonatal stress disrupts the glymphatic system development and increases the susceptibility to Parkinson's disease in later life.

ABSTRACT:

Introduction

Neonatal stress disrupts brain development and increases the risk of neurological disorders later in life. However, the impact of neonatal stress on the development of the glymphatic system and susceptibility to Parkinson's disease (PD) remains largely unknown.

Methods

Neonatal maternal deprivation (NMD) was performed on mice for 14 consecutive days to model chronic neonatal stress. Adeno-associated virus expressing A53T-α-synuclein (α-syn) was injected into the substantia nigra to establish PD model mice. Glymphatic activity was determined using in vivo magnetic resonance imaging, ex vivo fluorescence imaging and microplate assay. The transcription and expression of aquaporin-4 (AQP4) and other molecules were evaluated by qPCR, western blotting, and immunofluor

SUBMITTER: Song J 

PROVIDER: S-EPMC10851323 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature