Protein production dynamics and physiological adaptation of recombinant Komagataella phaffii at near-zero growth rates.

ABSTRACT:

Background

Specific productivity (q_P) in yeast correlates with growth, typically peaking at intermediate or maximum specific growth rates (μ). Understanding the factors limiting productivity at extremely low μ might reveal decoupling strategies, but knowledge of production dynamics and physiology in such conditions is scarce. Retentostats, a type of continuous cultivation, enable the well-controlled transition to near-zero µ through the combined retention of biomass and limited substrate supply. Recombinant Komagataella phaffii (syn Pichia pastoris) secreting a bivalent single domain antibody (VHH) was cultivated in aerobic, glucose-limited retentostats to investigate recombinant protein production dynamics and broaden our understanding of relevant physiological adaptati

SUBMITTER: Rebnegger C 

PROVIDER: S-EPMC10851509 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature