Effects of certain pre-analytical factors on the performance of plasma phospho-tau217.

ABSTRACT:

Introduction

Pre-analytical factors can cause substantial variability in the measurements of cerebrospinal fluid (CSF) and plasma biomarkers of Alzheimer's disease (AD). However, their effects on the performance of one of the most promising plasma AD biomarkers, phosphorylated tau (p-tau)217, are not known.

Methods

We included 50 amyloid-β positive (Aβ⁺) and 50 Aβ^- participants from the Swedish BioFINDER-1 study. Plasma and CSF p-tau217 were measured using an immunoassay developed by Lilly Research Laboratories. We examined the effect of four plasma handling conditions, i.e., (1) thawing at room temperature (RT) with no centrifugation, (2) thawing at RT followed by centrifugation, (3) thawing on ice with no centrifugation, and (4) thawing on ice followe

SUBMITTER: Bali D 

PROVIDER: S-EPMC10851521 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature