Class switching is differentially regulated in RBC alloimmunization and vaccination.

ABSTRACT:

Background

Studies of human patients have shown that most anti-RBC alloantibodies are IgG1 or IgG3 subclasses, although it is unclear why transfused RBCs preferentially drive these subclasses over others. Though mouse models allow for the mechanistic exploration of class-switching, previous studies of RBC alloimmunization in mice have focused more on the total IgG response than the relative distribution, abundance, or mechanism of IgG subclass generation. Given this major gap, we compared the IgG subclass distribution generated in response to transfused RBCs relative to protein in alum vaccination, and determined the role of STAT6 in their generation.

Study design and methods

WT mice were either immunized with Alum/HEL-OVA or transfused with HOD RBCs and levels of anti-HEL I

SUBMITTER: Prakash A 

PROVIDER: S-EPMC10851675 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature