Plasma ddPCR for the detection of MET amplification in advanced NSCLC patients: a comparative real-world study.

ABSTRACT:

Background

Mesenchymal-epithelial transition (MET) amplification is a crucial oncogenic driver and a resistance mechanism to epidermal growth factor receptor tyrosine kinase inhibitors (TKIs) of non-small-cell lung cancer (NSCLC). Fluorescence in situ hybridization (FISH) is the gold standard for MET amplification detection. However, it is inapplicable when tissue samples are unavailable.

Objective

This study assessed the performance of plasma droplet digital polymerase chain reaction (ddPCR) in MET amplification detection in NSCLC patients.

Design and methods

A total of 87 NSCLC patients were enrolled, and 94 paired tissue and plasma samples were analyzed for the concordance between FISH and plasma ddPCR/tissue next-generation sequencing (

SUBMITTER: Su JW 

PROVIDER: S-EPMC10851729 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature