General Principles and Limitations for Detection of RNA Modifications by Sequencing.

ABSTRACT: Among the many analytical methods applied to RNA modifications, a particularly pronounced surge has occurred in the past decade in the field of modification mapping. The occurrence of modifications such as m⁶A in mRNA, albeit known since the 1980s, became amenable to transcriptome-wide analyses through the advent of next-generation sequencing techniques in a rather sudden manner. The term "mapping" here refers to detection of RNA modifications in a sequence context, which has a dramatic impact on the interpretation of biological functions. As a consequence, an impressive number of mapping techniques were published, most in the perspective of what now has become known as "epitranscriptomics". While more and more different modifications were reported to occur in mRNA, conflicting

SUBMITTER: Motorin Y 

PROVIDER: S-EPMC10851944 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature