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Terbium-149 production: a focus on yield and quality improvement towards preclinical application.

ABSTRACT: Terbium-149 (T_1/2 = 4.1 h, E_α = 3.98 MeV (16.7%), 28 µm range in tissue) is a radionuclide with potential for targeted alpha therapy. Due to the negligible emission of α-emitting daughter nuclides, toxicity to healthy tissue may be reduced in comparison with other α-particle emitters. In this study, terbium-149 was produced via 1.4 GeV proton irradiation of a tantalum target at the CERN-ISOLDE facility. The spallation products were mass separated and implanted on zinc-coated foils and, later, radiochemically processed. Terbium-149 was separated from the co-produced isobaric radioisotopes and the zinc coating from the implantation foil, using cation-exchange and extraction chromatographic techniques, respectively. At the end of separation, up to 260 MBq terbium-149 were obtained with > 99% radionuclidic purity. Radiolabeling experiments were performed with DOTATATE, achieving 50 MBq/nmol apparent molar activity with radiochemical purity > 99%. The chemical purity was determined by inductively coupled plasma-mass spectrometry measurements, which showed lead, copper, iron and zinc only at ppb level. The radiolabeling of the somatostatin analogue DOTATATE with [¹⁴⁹Tb]TbCl₃ and the subsequent in vivo PET/CT scans conducted in xenografted mice, showing good tumor uptake, further demonstrated product quality and its ability to be used in a preclinical setting.

SUBMITTER: Favaretto C

PROVIDER: S-EPMC10853284 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature

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Terbium-149 production: a focus on yield and quality improvement towards preclinical application.

Favaretto C C Grundler P V PV Talip Z Z Köster U U Johnston K K Busslinger S D SD Sprung P P Hillhouse C C CC Eichler R R Schibli R R Müller C C van der Meulen N P NP

Scientific reports 20240208 1

Terbium-149 (T<sub>1/2</sub> = 4.1 h, E<sub>α</sub> = 3.98 MeV (16.7%), 28 µm range in tissue) is a radionuclide with potential for targeted alpha therapy. Due to the negligible emission of α-emitting daughter nuclides, toxicity to healthy tissue may be reduced in comparison with other α-particle emitters. In this study, terbium-149 was produced via 1.4 GeV proton irradiation of a tantalum target at the CERN-ISOLDE facility. The spallation products were mass separated and implanted on zinc-coate ...[more]

PMID: 38332245

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Dataset Information

Terbium-149 production: a focus on yield and quality improvement towards preclinical application.

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Terbium-149 production: a focus on yield and quality improvement towards preclinical application.

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