Aryl hydrocarbon receptor and IL-13 signaling crosstalk in human keratinocytes and atopic dermatitis.

ABSTRACT:

Introduction

Atopic dermatitis (AD) is an allergic skin disease mediated by skin barrier impairment and IL-13-driven immune response. Activation of the aryl hydrocarbon receptor (AHR) has shown promise in early clinical trials for AD; however, the mechanism by which AHR partially ameliorates AD is not well known.

Methods

Gene expression data from human biopsies were analyzed, and compared to gene expression from RNA-sequencing in our in-vitro HaCaT cell model system. Western blot, ELISA qRT-PCR were used to further explore the relationship between AHR and IL-13 signaling in HaCaT cells.

Results

The AHR target gene CYP1A1 was decreased in lesional skin compared with healthy control skin (p = 4.30 × 10^-9). Single-cell RNA sequencing (scRN

SUBMITTER: Proper SP 

PROVIDER: S-EPMC10853333 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature