CircBRIP1: a plasma diagnostic marker for non-small-cell lung cancer.

ABSTRACT:

Background

Circular RNA (circRNA), which has been demonstrated in studies to be abundantly prevalent in tumor cells and bodily fluids and to play a significant role in tumors, has the potential for biological markers to be used to assist tumor diagnosis. This study mainly discusses the potential of circBRIP1 as a biomarker for diagnosing non-small-cell lung cancer (NSCLC).

Methods

First, high-throughput sequencing screened the differentially expressed circBRIP1, and real-time fluorescence quantitative PCR (qRT-PCR) verified its expression in NSCLC. Next, sanger sequencing, agarose gel electrophoresis, RNase R assay, and fluorescence in situ hybridization (FISH) were used to verify its molecular characteristics. The diagnostic value was analyzed by the subject operating chara

SUBMITTER: Fan X 

PROVIDER: S-EPMC10853360 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature