Role of G protein-coupled receptor kinases (GRKs) in β₂ -adrenoceptor-mediated glucose uptake.

ABSTRACT: Truncation of the C-terminal tail of the β₂ -AR, transfection of βARKct or over-expression of a kinase-dead GRK mutant reduces isoprenaline-stimulated glucose uptake, indicating that GRK is important for this response. We explored whether phosphorylation of the β₂ -AR by GRK2 has a role in glucose uptake or if this response is related to the role of GRK2 as a scaffolding protein. CHO-GLUT4myc cells expressing wild-type and mutant β₂ -ARs were generated and receptor affinity for [³ H]-CGP12177A and density of binding sites determined together with the affinity of isoprenaline and BRL37344. Following receptor activation by β₂ -AR agonists, cAMP accumulation, GLUT4 translocation, [³ H]-2-deoxyglucose uptake, and β₂ -

SUBMITTER: Ham S 

PROVIDER: S-EPMC10853676 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature