An Alternating Current Electroosmotic Flow-Based Ultrasensitive Electrochemiluminescence Microfluidic System for Ultrafast Monitoring, Detection of Proteins/miRNAs in Unprocessed Samples.

ABSTRACT: Early diagnosis of acute diseases is restricted by the sensitivity and complex process of sample treatment. Here, an ultrasensitive, rapid, and portable electrochemiluminescence-microfluidic (ECL-M) system is described via sandwich-type immunoassay and surface plasmonic resonance (SPR) assay. Using a sandwich immunoreaction approach, the ECL-M system employs cardiac troponin-I antigen (cTnI) as a detection model with a Ru@SiO₂ NPs labeled antibody as the signal probe. For miR-499-5p detection, gold nanoparticles generate SPR effects to enhance Ru(bpy)₃ ²⁺ ECL signals. The system based on alternating current (AC) electroosmotic flow achieves an LOD of 2 fg mL^-1 for cTnI in 5 min and 10 aM for miRNAs in 10 min at room temperature. The point-of-care

SUBMITTER: Xiong H 

PROVIDER: S-EPMC10853704 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature