Structure of the WYL-domain containing transcription activator, DriD, in complex with ssDNA effector and DNA target site.

ABSTRACT: Transcription regulators play central roles in orchestrating responses to changing environmental conditions. Recently the Caulobacter crescentus transcription activator DriD, which belongs to the newly defined WYL-domain family, was shown to regulate DNA damage responses independent of the canonical SOS pathway. However, the molecular mechanisms by which DriD and other WYL-regulators sense environmental signals and recognize DNA are not well understood. We showed DriD DNA-binding is triggered by its interaction with ssDNA, which is produced during DNA damage. Here we describe the structure of the full-length C. crescentus DriD bound to both target DNA and effector ssDNA. DriD consists of an N-terminal winged-HTH (wHTH) domain, linker region, three-helix bundle, WYL-domain and C-terminal WC

SUBMITTER: Schumacher MA 

PROVIDER: S-EPMC10853764 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature