Replication elongates short DNA, reduces sequence bias and develops trimer structure.

ABSTRACT: The origin of molecular evolution required the replication of short oligonucleotides to form longer polymers. Prebiotically plausible oligonucleotide pools tend to contain more of some nucleobases than others. It has been unclear whether this initial bias persists and how it affects replication. To investigate this, we examined the evolution of 12-mer biased short DNA pools using an enzymatic model system. This allowed us to study the long timescales involved in evolution, since it is not yet possible with currently investigated prebiotic replication chemistries. Our analysis using next-generation sequencing from different time points revealed that the initial nucleotide bias of the pool disappeared in the elongated pool after isothermal replication. In contrast, the nucleotide composition

SUBMITTER: Calaca Serrao A 

PROVIDER: S-EPMC10853772 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature