Enhancing circuit stability under growth feedback with supplementary repressive regulation.

ABSTRACT: The field of synthetic biology and biosystems engineering increasingly acknowledges the need for a holistic design approach that incorporates circuit-host interactions into the design process. Engineered circuits are not isolated entities but inherently entwined with the dynamic host environment. One such circuit-host interaction, 'growth feedback', results when modifications in host growth patterns influence the operation of gene circuits. The growth-mediated effects can range from growth-dependent elevation in protein/mRNA dilution rate to changes in resource reallocation within the cell, which can lead to complete functional collapse in complex circuits. To achieve robust circuit performance, synthetic biologists employ a variety of control mechanisms to stabilize and insulate circuit b

SUBMITTER: Stone A 

PROVIDER: S-EPMC10853785 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature