Electroacupuncture ameliorates neuroinflammation by inhibiting TRPV4 channel in ischemic stroke.

ABSTRACT:

Aims

We investigated the potential mechanisms underlying the therapeutic efficacy of electroacupuncture (EA) at the Shuigou (GV26) and Baihui (GV20) acupoints in the treatment of ischemic stroke.

Methods

We assessed the therapeutic effects of EA on MCAO mice through behavioral studies and TTC staining. Various techniques, such as RT-PCR, immunofluorescence, and Western blots, were employed to evaluate the activation and polarization of microglia/macrophages, and changes in the TRPV4 ion channel. We used the TRPV4 antagonist GSK2193874 (GSK219) to verify the involvement of TRPV4 in the therapeutic effects of EA.

Results

EA effectively improved neurological impairments and reduced cerebral infarction volume in MCAO mice. It suppressed activated microglia/macrophages and

SUBMITTER: Ren X 

PROVIDER: S-EPMC10853892 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature